|B.Pharma| INSTRUMENTAL METHODS OF ANALYSIS Unit-1st Best Handwritten Notes Free PDF Download |7th Semester|

INSTRUMENTAL METHODS OF ANALYSIS unit-1st handwritten notes |7th Semester|

UV Visible spectroscopy Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations. Instrumentation – Sources of radiation, wavelength selectors, sample cells, detectors- Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode. Applications – Spectrophotometric titrations, Single component and multi component analysis Fluorimetry Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation and applications

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Index

• UV Visible spectroscopy
• Electronic transitions
•  chromophores, auxochromes
• spectral shifts, solvent effect on
• absorption spectra
• Beer and Lambert’s law
• Derivation and deviations.
• Instrumentation – Sources of radiation
• wavelength selectors
•  sample cells, detectors-
• Photo tube, Photomultiplier tube
• Photo voltaic cell, Silicon Photodiode.
• Applications – Spectrophotometric titrations
• Single component and multi component
• analysis
• Fluorimetry
• Theory, Concepts of singlet
• doublet and triplet electronic states
• internal and external
• conversions
• factors affecting fluorescence
• quenching
• instrumentation and
• applications

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