INSTRUMENTAL METHODS OF ANALYSIS unit-1st handwritten notes |7th Semester|
UV Visible spectroscopy Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations. Instrumentation – Sources of radiation, wavelength selectors, sample cells, detectors- Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode. Applications – Spectrophotometric titrations, Single component and multi component analysis Fluorimetry Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation and applications
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Index
- UV Visible spectroscopy
- Electronic transitions
- chromophores, auxochromes
- spectral shifts, solvent effect on
- absorption spectra
- Beer and Lambert’s law
- Derivation and deviations.
- Instrumentation – Sources of radiation
- wavelength selectors
- sample cells, detectors-
- Photo tube, Photomultiplier tube
- Photo voltaic cell, Silicon Photodiode.
- Applications – Spectrophotometric titrations
- Single component and multi component
- analysis
- Fluorimetry
- Theory, Concepts of singlet
- doublet and triplet electronic states
- internal and external
- conversions
- factors affecting fluorescence
- quenching
- instrumentation and
- applications
